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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which results in chromosome instability, and thus might contribute to tumorigenesis. This protein was identified to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital part in advertising and marketing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been explained. Two more splice variants have been noted but thorough nucleotide sequence info is not really still offered.
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Blocking peptides are peptides that bind exclusively to the target antibody and block antibody binding. These peptide normally contains the epitope recognized through the antibody.
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This guideline outlines further achievable optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
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This manual outlines further probable optimization of mobile and immunoassay parameters to be certain the absolute best outcomes are...
Antibodies sure to the blocking peptide no longer bind on the epitope within the concentrate on protein. By comparing the staining with the blocked antibody versus the antibody by yourself, you can see which staining is certain.
Concentrate on Info Cyclin E belongs to your highly conserved cyclin household, whose users are characterised by a remarkable periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins exhibit distinct expression and degradation patterns which contribute towards the temporal coordination of every mitotic function. Cyclin E types a complex with and functions as being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates on the G1-S stage boundary and it is degraded as cells progress via S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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